Understanding The TessArae Technology Advantage
TessArae’s proprietary targeted sequencing technology utilizes a unique "sequencing-by-hybridization" strategy. Eight probes are created for each nucleotide pair to be sequenced–four each for the forward and reverse strands. These two groups of four probes are then tiled together on the array as a probe set.
Each of the probes is 25 nucleotides in length, and varies only at the center with an A, G, C or T at that position. The probe sequences are identical and complementary to the sequence of the target from the specimen to be analyzed.
Heterozygous Alleles Our unique design strategy targets both the wildtype and mutant sequences for every locus tested. This allows a target containing a heterozygous mutation to be accurately detected through hybridization of both the wildtype and mutant alleles to their complementary probes.
Insertions and Deletions For insertion and deletion mutations, our design approach targets breakpoint and junction sequences, ensuring accurate detection of known heterozygous and homozygous indels.
Novel Single Nucleotide Changes Since mutant alleles are targeted in addition to wildtype alleles, any novel single nucleotide mutation or polymorphism can be detected at any position in the analyzed genes. Similar to the detection of a heterozygous allele, the target containing a novel mutation will hybridize to its complementary probe set, just to a different probe in the probe set.
Automated Analysis When the run is complete, the resulting data file from the assay is uploaded to TessArae via a secure, encrypted web portal for analysis using the TessArae GeneCipher™ algorithm. This exclusive algorithm generates an automated list, gene-by-gene, mutation-by-mutation, of the results within minutes of completing the assay.
The TessArae Technology Platform enables a host of new approaches for designing robust, highly sensitive, and specific targeted sequencing assays with the GeneCipher algorithm–helping you to detect hundreds of homozygous and heterozygous alleles.